The metabolite chemotype of Nicotiana benthamiana transiently expressing artemisinin biosynthetic pathway genes is a function of CYP71AV1 type and relative gene dosage.
Identifieur interne : 000441 ( Main/Exploration ); précédent : 000440; suivant : 000442The metabolite chemotype of Nicotiana benthamiana transiently expressing artemisinin biosynthetic pathway genes is a function of CYP71AV1 type and relative gene dosage.
Auteurs : Hieng-Ming Ting [Pays-Bas] ; Bo Wang ; Anna-Margareta Rydén ; Lotte Woittiez ; Teun Van Herpen ; Francel W A. Verstappen ; Carolien Ruyter-Spira ; Jules Beekwilder ; Harro J. Bouwmeester ; Alexander Van Der KrolSource :
- The New phytologist [ 1469-8137 ] ; 2013.
Descripteurs français
- KwdFr :
- Agrobacterium (métabolisme), Artémisinines (métabolisme), Chromatographie en phase liquide à haute performance (MeSH), Données de séquences moléculaires (MeSH), Dosage génique (MeSH), Feuilles de plante (métabolisme), Fractions subcellulaires (métabolisme), Glutathion (métabolisme), Glycosylation (MeSH), Modèles biologiques (MeSH), Protéines végétales (composition chimique), Protéines végétales (génétique), Protéines végétales (métabolisme), Régulation de l'expression des gènes végétaux (MeSH), Réticulum endoplasmique (métabolisme), Spectrométrie de masse (MeSH), Séquence d'acides aminés (MeSH), Tabac (génétique), Tabac (métabolisme), Transport des protéines (MeSH), Voies de biosynthèse (génétique).
- MESH :
- composition chimique : Protéines végétales.
- génétique : Protéines végétales, Tabac, Voies de biosynthèse.
- métabolisme : Agrobacterium, Artémisinines, Feuilles de plante, Fractions subcellulaires, Glutathion, Protéines végétales, Réticulum endoplasmique, Tabac.
- Chromatographie en phase liquide à haute performance, Données de séquences moléculaires, Dosage génique, Glycosylation, Modèles biologiques, Régulation de l'expression des gènes végétaux, Spectrométrie de masse, Séquence d'acides aminés, Transport des protéines.
English descriptors
- KwdEn :
- Agrobacterium (metabolism), Amino Acid Sequence (MeSH), Artemisinins (metabolism), Biosynthetic Pathways (genetics), Chromatography, High Pressure Liquid (MeSH), Endoplasmic Reticulum (metabolism), Gene Dosage (MeSH), Gene Expression Regulation, Plant (MeSH), Glutathione (metabolism), Glycosylation (MeSH), Mass Spectrometry (MeSH), Models, Biological (MeSH), Molecular Sequence Data (MeSH), Plant Leaves (metabolism), Plant Proteins (chemistry), Plant Proteins (genetics), Plant Proteins (metabolism), Protein Transport (MeSH), Subcellular Fractions (metabolism), Tobacco (genetics), Tobacco (metabolism).
- MESH :
- chemical , chemistry : Plant Proteins.
- chemical , genetics : Plant Proteins.
- chemical , metabolism : Artemisinins, Glutathione, Plant Proteins.
- genetics : Biosynthetic Pathways, Tobacco.
- metabolism : Agrobacterium, Endoplasmic Reticulum, Plant Leaves, Subcellular Fractions, Tobacco.
- Amino Acid Sequence, Chromatography, High Pressure Liquid, Gene Dosage, Gene Expression Regulation, Plant, Glycosylation, Mass Spectrometry, Models, Biological, Molecular Sequence Data, Protein Transport.
Abstract
Artemisia annua, which produces the anti-malaria compound artemisinin, occurs as high-artemisinin production (HAP) and low-artemisinin production (LAP) chemotypes. Understanding the basis of the difference between these chemotypes would assist breeding and optimising artemisinin biosynthesis. Here we present a systematic comparison of artemisinin biosynthesis genes that may be involved in determining the chemotype (CYP71AV1, DBR2 and ALDH1). These genes were isolated from the two chemotypes and characterized using transient expression in planta. The enzyme activity of DBR2 and ALDH1 from the two chemotypes did not differ, but structural differences in CYP71AV1 from LAP and HAP chemotypes (AMOLAP and AMOHAP, respectively) resulted in altered enzyme activity. AMOLAP displays a seven amino acids N-terminal extension compared with AMOHAP. The GFP fusion of both proteins show equal localization to the ER but AMOHAP may have reduced stability. Upon transient expression in Nicotiana benthamiana, AMOLAP displayed a higher enzyme activity than AMOHAP. However, expression in combination with the other pathway genes also resulted in a qualitatively different product profile ('chemotype'); that is, in a shift in the ratio between the unsaturated and saturated (dihydro) branch of the pathway.
DOI: 10.1111/nph.12274
PubMed: 23638869
Affiliations:
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Verstappen</name></author><author><name sortKey="Ruyter Spira, Carolien" sort="Ruyter Spira, Carolien" uniqKey="Ruyter Spira C" first="Carolien" last="Ruyter-Spira">Carolien Ruyter-Spira</name></author><author><name sortKey="Beekwilder, Jules" sort="Beekwilder, Jules" uniqKey="Beekwilder J" first="Jules" last="Beekwilder">Jules Beekwilder</name></author><author><name sortKey="Bouwmeester, Harro J" sort="Bouwmeester, Harro J" uniqKey="Bouwmeester H" first="Harro J" last="Bouwmeester">Harro J. Bouwmeester</name></author><author><name sortKey="Van Der Krol, Alexander" sort="Van Der Krol, Alexander" uniqKey="Van Der Krol A" first="Alexander" last="Van Der Krol">Alexander Van Der Krol</name></author></analytic><series><title level="j">The New phytologist</title><idno type="eISSN">1469-8137</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Agrobacterium (metabolism)</term><term>Amino Acid Sequence (MeSH)</term><term>Artemisinins (metabolism)</term><term>Biosynthetic Pathways (genetics)</term><term>Chromatography, High Pressure Liquid (MeSH)</term><term>Endoplasmic Reticulum (metabolism)</term><term>Gene Dosage (MeSH)</term><term>Gene Expression Regulation, Plant (MeSH)</term><term>Glutathione (metabolism)</term><term>Glycosylation (MeSH)</term><term>Mass Spectrometry (MeSH)</term><term>Models, Biological (MeSH)</term><term>Molecular Sequence Data (MeSH)</term><term>Plant Leaves (metabolism)</term><term>Plant Proteins (chemistry)</term><term>Plant Proteins (genetics)</term><term>Plant Proteins (metabolism)</term><term>Protein Transport (MeSH)</term><term>Subcellular Fractions (metabolism)</term><term>Tobacco (genetics)</term><term>Tobacco (metabolism)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Agrobacterium (métabolisme)</term><term>Artémisinines (métabolisme)</term><term>Chromatographie en phase liquide à haute performance (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Dosage génique (MeSH)</term><term>Feuilles de plante (métabolisme)</term><term>Fractions subcellulaires (métabolisme)</term><term>Glutathion (métabolisme)</term><term>Glycosylation (MeSH)</term><term>Modèles biologiques (MeSH)</term><term>Protéines végétales (composition chimique)</term><term>Protéines végétales (génétique)</term><term>Protéines végétales (métabolisme)</term><term>Régulation de l'expression des gènes végétaux (MeSH)</term><term>Réticulum endoplasmique (métabolisme)</term><term>Spectrométrie de masse (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term><term>Tabac (génétique)</term><term>Tabac (métabolisme)</term><term>Transport des protéines (MeSH)</term><term>Voies de biosynthèse (génétique)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Plant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Artemisinins</term><term>Glutathione</term><term>Plant Proteins</term></keywords><keywords scheme="MESH" qualifier="composition chimique" xml:lang="fr"><term>Protéines végétales</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Biosynthetic Pathways</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines végétales</term><term>Tabac</term><term>Voies de biosynthèse</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Agrobacterium</term><term>Endoplasmic Reticulum</term><term>Plant Leaves</term><term>Subcellular Fractions</term><term>Tobacco</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Agrobacterium</term><term>Artémisinines</term><term>Feuilles de plante</term><term>Fractions subcellulaires</term><term>Glutathion</term><term>Protéines végétales</term><term>Réticulum endoplasmique</term><term>Tabac</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Chromatography, High Pressure Liquid</term><term>Gene Dosage</term><term>Gene Expression Regulation, Plant</term><term>Glycosylation</term><term>Mass Spectrometry</term><term>Models, Biological</term><term>Molecular Sequence Data</term><term>Protein Transport</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Chromatographie en phase liquide à haute performance</term><term>Données de séquences moléculaires</term><term>Dosage génique</term><term>Glycosylation</term><term>Modèles biologiques</term><term>Régulation de l'expression des gènes végétaux</term><term>Spectrométrie de masse</term><term>Séquence d'acides aminés</term><term>Transport des protéines</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Artemisia annua, which produces the anti-malaria compound artemisinin, occurs as high-artemisinin production (HAP) and low-artemisinin production (LAP) chemotypes. Understanding the basis of the difference between these chemotypes would assist breeding and optimising artemisinin biosynthesis. Here we present a systematic comparison of artemisinin biosynthesis genes that may be involved in determining the chemotype (CYP71AV1, DBR2 and ALDH1). These genes were isolated from the two chemotypes and characterized using transient expression in planta. The enzyme activity of DBR2 and ALDH1 from the two chemotypes did not differ, but structural differences in CYP71AV1 from LAP and HAP chemotypes (AMOLAP and AMOHAP, respectively) resulted in altered enzyme activity. AMOLAP displays a seven amino acids N-terminal extension compared with AMOHAP. The GFP fusion of both proteins show equal localization to the ER but AMOHAP may have reduced stability. Upon transient expression in Nicotiana benthamiana, AMOLAP displayed a higher enzyme activity than AMOHAP. However, expression in combination with the other pathway genes also resulted in a qualitatively different product profile ('chemotype'); that is, in a shift in the ratio between the unsaturated and saturated (dihydro) branch of the pathway.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23638869</PMID><DateCompleted><Year>2014</Year><Month>01</Month><Day>15</Day></DateCompleted><DateRevised><Year>2020</Year><Month>09</Month><Day>30</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1469-8137</ISSN><JournalIssue CitedMedium="Internet"><Volume>199</Volume><Issue>2</Issue><PubDate><Year>2013</Year><Month>Jul</Month></PubDate></JournalIssue><Title>The New phytologist</Title><ISOAbbreviation>New Phytol</ISOAbbreviation></Journal><ArticleTitle>The metabolite chemotype of Nicotiana benthamiana transiently expressing artemisinin biosynthetic pathway genes is a function of CYP71AV1 type and relative gene dosage.</ArticleTitle><Pagination><MedlinePgn>352-66</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1111/nph.12274</ELocationID><Abstract><AbstractText>Artemisia annua, which produces the anti-malaria compound artemisinin, occurs as high-artemisinin production (HAP) and low-artemisinin production (LAP) chemotypes. Understanding the basis of the difference between these chemotypes would assist breeding and optimising artemisinin biosynthesis. Here we present a systematic comparison of artemisinin biosynthesis genes that may be involved in determining the chemotype (CYP71AV1, DBR2 and ALDH1). These genes were isolated from the two chemotypes and characterized using transient expression in planta. The enzyme activity of DBR2 and ALDH1 from the two chemotypes did not differ, but structural differences in CYP71AV1 from LAP and HAP chemotypes (AMOLAP and AMOHAP, respectively) resulted in altered enzyme activity. AMOLAP displays a seven amino acids N-terminal extension compared with AMOHAP. The GFP fusion of both proteins show equal localization to the ER but AMOHAP may have reduced stability. Upon transient expression in Nicotiana benthamiana, AMOLAP displayed a higher enzyme activity than AMOHAP. However, expression in combination with the other pathway genes also resulted in a qualitatively different product profile ('chemotype'); that is, in a shift in the ratio between the unsaturated and saturated (dihydro) branch of the pathway.</AbstractText><CopyrightInformation>© 2013 The Authors. New Phytologist © 2013 New Phytologist Trust.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Ting</LastName><ForeName>Hieng-Ming</ForeName><Initials>HM</Initials><AffiliationInfo><Affiliation>Laboratory of Plant Physiology, Wageningen University, Droevendaalsesteeg 1, 6708 PB, Wageningen, the Netherlands.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Bo</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Rydén</LastName><ForeName>Anna-Margareta</ForeName><Initials>AM</Initials></Author><Author ValidYN="Y"><LastName>Woittiez</LastName><ForeName>Lotte</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>van Herpen</LastName><ForeName>Teun</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Verstappen</LastName><ForeName>Francel W A</ForeName><Initials>FW</Initials></Author><Author ValidYN="Y"><LastName>Ruyter-Spira</LastName><ForeName>Carolien</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Beekwilder</LastName><ForeName>Jules</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Bouwmeester</LastName><ForeName>Harro J</ForeName><Initials>HJ</Initials></Author><Author ValidYN="Y"><LastName>van der Krol</LastName><ForeName>Alexander</ForeName><Initials>A</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>05</Month><Day>02</Day></ArticleDate></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>New Phytol</MedlineTA><NlmUniqueID>9882884</NlmUniqueID><ISSNLinking>0028-646X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D037621">Artemisinins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D010940">Plant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C520462">dihydroartemisinic acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>53N99527G7</RegistryNumber><NameOfSubstance UI="C047721">artemisic acid</NameOfSubstance></Chemical><Chemical><RegistryNumber>9RMU91N5K2</RegistryNumber><NameOfSubstance UI="C031327">artemisinine</NameOfSubstance></Chemical><Chemical><RegistryNumber>GAN16C9B8O</RegistryNumber><NameOfSubstance UI="D005978">Glutathione</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D060054" MajorTopicYN="N">Agrobacterium</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D037621" MajorTopicYN="N">Artemisinins</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D053898" MajorTopicYN="N">Biosynthetic Pathways</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002851" MajorTopicYN="N">Chromatography, High Pressure Liquid</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004721" MajorTopicYN="N">Endoplasmic Reticulum</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D018628" MajorTopicYN="Y">Gene Dosage</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018506" MajorTopicYN="Y">Gene Expression Regulation, Plant</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005978" MajorTopicYN="N">Glutathione</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006031" MajorTopicYN="N">Glycosylation</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013058" MajorTopicYN="N">Mass Spectrometry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008954" MajorTopicYN="N">Models, Biological</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D018515" MajorTopicYN="N">Plant Leaves</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D010940" MajorTopicYN="N">Plant Proteins</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D021381" MajorTopicYN="N">Protein Transport</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013347" MajorTopicYN="N">Subcellular Fractions</DescriptorName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014026" MajorTopicYN="N">Tobacco</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2012</Year><Month>11</Month><Day>27</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>03</Month><Day>10</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>5</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>5</Month><Day>4</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>1</Month><Day>16</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23638869</ArticleId><ArticleId IdType="doi">10.1111/nph.12274</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Pays-Bas</li></country><region><li>Gueldre (province)</li></region><settlement><li>Wageningue</li></settlement><orgName><li>Université de Wageningue</li></orgName></list><tree><noCountry><name sortKey="Beekwilder, Jules" sort="Beekwilder, Jules" uniqKey="Beekwilder J" first="Jules" last="Beekwilder">Jules Beekwilder</name><name sortKey="Bouwmeester, Harro J" sort="Bouwmeester, Harro J" uniqKey="Bouwmeester H" first="Harro J" last="Bouwmeester">Harro J. Bouwmeester</name><name sortKey="Ruyter Spira, Carolien" sort="Ruyter Spira, Carolien" uniqKey="Ruyter Spira C" first="Carolien" last="Ruyter-Spira">Carolien Ruyter-Spira</name><name sortKey="Ryden, Anna Margareta" sort="Ryden, Anna Margareta" uniqKey="Ryden A" first="Anna-Margareta" last="Rydén">Anna-Margareta Rydén</name><name sortKey="Van Der Krol, Alexander" sort="Van Der Krol, Alexander" uniqKey="Van Der Krol A" first="Alexander" last="Van Der Krol">Alexander Van Der Krol</name><name sortKey="Van Herpen, Teun" sort="Van Herpen, Teun" uniqKey="Van Herpen T" first="Teun" last="Van Herpen">Teun Van Herpen</name><name sortKey="Verstappen, Francel W A" sort="Verstappen, Francel W A" uniqKey="Verstappen F" first="Francel W A" last="Verstappen">Francel W A. Verstappen</name><name sortKey="Wang, Bo" sort="Wang, Bo" uniqKey="Wang B" first="Bo" last="Wang">Bo Wang</name><name sortKey="Woittiez, Lotte" sort="Woittiez, Lotte" uniqKey="Woittiez L" first="Lotte" last="Woittiez">Lotte Woittiez</name></noCountry><country name="Pays-Bas"><region name="Gueldre (province)"><name sortKey="Ting, Hieng Ming" sort="Ting, Hieng Ming" uniqKey="Ting H" first="Hieng-Ming" last="Ting">Hieng-Ming Ting</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|texte= The metabolite chemotype of Nicotiana benthamiana transiently expressing artemisinin biosynthetic pathway genes is a function of CYP71AV1 type and relative gene dosage.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:23638869" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a AgrobacTransV1
| This area was generated with Dilib version V0.6.38. |  |